**** The Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit is a specialized tool tailored for the quantitative detection of Hepatocyte Growth Factor levels in various biological samples from rabbits. Hepatocyte Growth Factor is a key protein involved in regulating cell growth, proliferation, and regeneration, particularly in the liver and other tissues in rabbits. Accurate measurement of HGF levels is pivotal for studying the role of this growth factor in liver health, tissue repair, and developmental processes.
This ELISA kit ensures precise and reliable results, offering exceptional sensitivity and specificity in quantifying HGF concentrations. With stringent quality assurance measures in place, the kit delivers robust performance and user-friendly protocols, making it an ideal choice for researchers investigating the molecular mechanisms underlying cell growth and regeneration in rabbit models.
Product Name:
Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit
SKU:
AEES00581
Target:
Rabbit HGF (Hepatocyte Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit HGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit HGF. You can calculate the concentration of Rabbit HGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
100-112
87-100
Average (%)
91
105
94
1:4
Range (%)
84-96
94-106
84-98
Average (%)
88
100
90
1:8
Range (%)
83-94
98-109
90-105
Average (%)
89
104
97
1:16
Range (%)
85-98
97-110
86-102
Average (%)
92
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.27
87.73
476.31
52.45
89.46
498.61
Standard deviation
2.95
3.64
20.05
2.87
4.51
17.7
C V (%)
6.11
4.15
4.21
5.47
5.04
3.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit HGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit HGF in samples. No significant cross-reactivity or interference between Rabbit HGF and analogues was observed.
