The Rabbit IFN-γ (Interferon Gamma) ELISA Kit is specifically designed for the quantitative detection of IFN-γ levels in various rabbit biological samples. Interferon gamma, also known as IFN-γ, is a critical pro-inflammatory cytokine that plays a crucial role in the immune response against viral, bacterial, and parasitic infections. IFN-γ is involved in various cellular activities, including immune cell activation, antigen presentation, and regulation of immune responses. Accurate measurement of IFN-γ levels is essential for understanding its role in immunity, infection, and inflammatory diseases.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results in the quantitative analysis of IFN-γ. Manufactured under strict quality control standards, the kit provides reliable performance and ease of use, making it an ideal choice for researchers investigating immune responses, infectious diseases, and inflammatory disorders.
Product Name:
Rabbit IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
AEES00574
Target:
Rabbit IFN-γ (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IFN-γ. You can calculate the concentration of Rabbit IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
86-98
89-102
Average (%)
95
92
95
1:4
Range (%)
89-104
83-95
85-98
Average (%)
96
89
91
1:8
Range (%)
89-104
82-93
83-97
Average (%)
96
88
90
1:16
Range (%)
89-102
81-93
82-97
Average (%)
95
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.97
85.79
461.52
49.68
87.5
456.6
Standard deviation
2.48
4.15
21.69
3.33
4.91
17.44
C V (%)
5.28
4.84
4.7
6.7
5.61
3.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IFN-γ in samples. No significant cross-reactivity or interference between Rabbit IFN-γ and analogues was observed.
