The Rabbit IL-8 ELISA Kit is specifically designed for the quantitative detection of Interleukin-8 (IL-8) levels in various rabbit biological samples.
IL-8 is a pivotal pro-inflammatory chemokine that orchestrates immune responses, stimulates chemotaxis, and regulates neutrophil recruitment during inflammation, infection, and injury. Precise measurement of IL-8 is indispensable for investigating inflammatory processes, immune modulation, and the pathogenesis of various diseases where inflammation is a key component. This ELISA kit offers remarkable sensitivity and specificity, ensuring accurate and reproducible results in IL-8 quantification. Manufactured under strict quality control measures, the kit delivers reliable performance and user-friendly protocols, making it an excellent tool for researchers investigating cellular signaling pathways, immune responses, and inflammatory conditions in rabbit models.
Product Name:
Rabbit IL-8 ELISA Kit
SKU:
AEES00577
Target:
Rabbit IL-8
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-8. You can calculate the concentration of Rabbit IL-8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
86-100
94-105
Average (%)
102
91
100
1:4
Range (%)
87-100
79-93
85-97
Average (%)
94
85
90
1:8
Range (%)
90-107
86-98
85-98
Average (%)
98
92
92
1:16
Range (%)
89-101
87-100
82-96
Average (%)
96
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.17
292.21
891.02
86.63
281.46
810.4
Standard deviation
6.14
13.53
34.93
5.29
16.55
44.33
C V (%)
6.38
4.63
3.92
6.11
5.88
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IL-8 in samples. No significant cross-reactivity or interference between Rabbit IL-8 and analogues was observed.
