The Rabbit MT (Melatonin) ELISA Kit is a highly specialized assay designed for the quantitative measurement of Melatonin levels in various biological samples from rabbits.
Melatonin is a critical hormone that regulates the sleep-wake cycle, circadian rhythms, and other physiological functions in rabbits. Accurate detection of Melatonin levels is crucial for investigating the role of this hormone in regulating various biological processes in rabbits. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reliable results in the measurement of Melatonin concentrations. With stringent quality control measures in place, the kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers studying circadian rhythms, sleep disorders, and neuroendocrine regulation in rabbits.
Product Name:
Rabbit MT (Melatonin) ELISA Kit
SKU:
AEES00580
Target:
Rabbit MT (Melatonin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
84-97
86-101
Average (%)
91
90
93
1:4
Range (%)
85-96
88-102
94-107
Average (%)
90
94
102
1:8
Range (%)
86-101
87-99
97-113
Average (%)
92
94
103
1:16
Range (%)
85-99
89-102
98-113
Average (%)
92
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.5
297.3
915.7
94.9
322.9
926.1
Standard deviation
5.7
17.5
33.9
6.5
14.5
35.2
C V (%)
5.73
5.89
3.7
6.85
4.49
3.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
