Rabbit Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit
The Rabbit Tissue Inhibitors of Metalloproteinase 4 (TIMP4) ELISA Kit is a specialized assay designed for the precise measurement of TIMP4 levels in rabbit tissues and biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for research purposes.TIMP4 is a key regulator of metalloproteinase activity, playing a critical role in tissue modeling and repair processes. Abnormal levels of TIMP4 have been linked to various diseases and conditions, highlighting its significance as a potential biomarker for understanding disease pathology and developing therapeutic strategies.
With its advanced technology and robust performance, the Rabbit TIMP4 ELISA Kit is an invaluable tool for researchers studying the role of TIMP4 in various biological processes and disease mechanisms. Get reliable and reproducible results with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Rabbit Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit
Product Code:
RBDL00077
Size:
96 Assays
Target:
TIMP4
Synonyms:
TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor of Metalloproteinases 4
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
