This kit is comprised by HRP conjugate, other reagents and ELISA Microtiter plate pre-coated withrecombinant Rabies Virus (RBV) antigen. Apply the principle of enzyme-linked immunoassay (ELISA)to detect RBV-Ab in serum of canine and cat. During the experiment, add control and samples into theELISA Microtiter plate, RBV-Ab will be bound with the antigen on the ELISA Microtiter plate. Thenhorseradish peroxidase (HRP) conjugate is added to each ELISA microtiter plate well, and substratereagent is added for color development, the blue signal by Enzyme catalysis is in positive correlation ofantibody content in sample. Measure the absorbance value of each well by using a microplate readerwith 450 nm (630 nm) wavelength, then we can judge whether RBV antibody exist in the sample.
