The Rad52 Antibody (CAB3077) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human RAD52 samples and is validated for use in a variety of applications, including Western blotting. By binding to RAD52 protein, this antibody allows for the detection and analysis of RAD52 in different cellular contexts, making it an ideal choice for studies in DNA repair mechanisms and cancer research.RAD52 is essential for homologous recombination, a process crucial for repairing double-strand breaks in DNA and maintaining genome stability.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rad52 Antibody
SKU:
CAB3077
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAD52, Rad52
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
46kDa
Observed MW:
50kDa
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Purification Method
Affinity purification
Gene ID
5893
RRID
AB_2764880
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad52 Rabbit pAb (CAB3077) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
