The Rad52 Monoclonal Antibody (CAB5186) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is optimized for detecting RAD52 protein in human samples and is suitable for use in Western blot assays.RAD52 is essential for maintaining genome stability and promoting DNA repair through homologous recombination, making it a critical factor in preventing genomic instability and tumorigenesis. By targeting RAD52, researchers can gain insights into the mechanisms underlying DNA repair pathways and identify potential therapeutic targets for cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Rad52 Monoclonal Antibody
SKU:
CAB5186
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1183
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAD52, DNA repair protein RAD52 homolog, Rad52
Positive Sample:
HeLa, 293T, PC-3
Cellular Localization:
Nucleus.
Calculated MW:
13kDa/15kDa/25kDa/46kDa
Observed MW:
46kDa
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Purification Method
Affinity purification
Gene ID
5893
RRID
AB_2863479
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad52 Rabbit mAb (CAB5186) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Plus Kit (AbGn00021) . Exposure time: 3min.
