The Rad52 Monoclonal Antibody (CAB5186) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. RRID AB_2863479 Gene ID 5893 Swiss Prot Synonym RAD52; DNA repair protein RAD52 homolog; Rad52
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Rad52 Monoclonal Antibody
SKU:
CAB5186
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1183
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAD52, DNA repair protein RAD52 homolog, Rad52
Positive Sample:
HeLa, 293T, PC-3
Cellular Localization:
Nucleus.
Calculated MW:
13kDa/15kDa/25kDa/46kDa
Observed MW:
46kDa
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. RRID AB_2863479 Gene ID 5893 Swiss Prot Synonym RAD52; DNA repair protein RAD52 homolog; Rad52
Purification Method:
Affinity purification
Gene ID:
5893
RRID:
AB_2863479
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad52 Rabbit mAb (CAB5186) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Plus Kit (AbGn00021) . Exposure time: 3min.
