The RAG1 Antibody (CAB12646) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RAG1 protein, this antibody enables the detection and analysis of RAG1 expression in various cell types, making it ideal for investigations in immunology and hematopoiesis research.RAG1, a key component of the RAG complex, plays a pivotal role in initiating the recombination process that generates functional T and B cell receptors.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
RAG1 Antibody
SKU:
CAB12646
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAG-1, RNF74, RAG1
Positive Sample:
Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
119kDa
Observed MW:
110kDa
The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Purification Method
Affinity purification
Gene ID
5896
RRID
AB_2759491
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse thymus, using RAG1 Rabbit pAb (CAB12646) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
