The RALGDS Antibody (CAB17963) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for RalGDS and is suitable for use in various applications, including Western blotting and immunohistochemistry.RalGDS is a guanine nucleotide exchange factor that activates the small GTPase Ral, leading to downstream signaling events that regulate cell proliferation, migration, and survival.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RALGDS Antibody
SKU:
CAB17963
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RGF, RGDS, RalGEF, RALGDS
Positive Sample:
U-87MG, Mouse ovary, Mouse lung
Cellular Localization:
Cytosol, Nucleus, Plasma Membrane.
Calculated MW:
101kDa
Observed MW:
110kDa
Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.
Purification Method
Affinity purification
Gene ID
5900
RRID
AB_2861765
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RALGDS Rabbit pAb (CAB17963) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
