Rat A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit
The Rat ADAMTS4 (A Disintegrin and Metalloproteinase with Thrombospondin-4) ELISA Kit is expertly designed for the precise measurement of ADAMTS4 levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers trustworthy and consistent outcomes, making it an excellent choice for various research endeavors.ADAMTS4 is a pivotal enzyme that plays a crucial role in extracellular matrix remodeling and cartilage metabolism.
Its dysregulation has been implicated in diseases such as osteoarthritis and rheumatoid arthritis, highlighting its significance as a biomarker for understanding these pathologies and exploring potential treatment options.Incorporate the Rat ADAMTS4 ELISA Kit into your research arsenal to delve deeper into the intricate mechanisms of cartilage degradation and to uncover novel insights into musculoskeletal disorders.
Product Name:
Rat A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit
Product Code:
RTDL00022
Size:
96 Assays
Target:
ADAMTS4
Synonyms:
ADMP-1, Aggrecanase-1
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
