Rat A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit
The Rat ADAMTS5 (A Disintegrin and Metalloproteinase with Thrombospondin-5) ELISA Kit is a powerful tool for measuring ADAMTS5 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications.ADAMTS5 is an important enzyme involved in the breakdown of extracellular matrix components, playing a role in tissue remodeling and inflammation. Dysregulation of ADAMTS5 has been linked to various diseases, including osteoarthritis and cardiovascular disorders, highlighting its significance as a potential biomarker for disease progression and treatment development.
With the Rat ADAMTS5 ELISA Kit, researchers can explore the role of ADAMTS5 in disease pathogenesis, paving the way for new diagnostic strategies and therapeutic interventions. Get reliable and insightful data with this innovative ELISA kit from AssayGenie.
Product Name:
Rat A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA Kit
Product Code:
RTDL00023
Size:
96 Assays
Target:
ADAMTS5
Synonyms:
ADMP-2, Aggrecanase-2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
