Rat ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit
The Rat ABCA1 (ATP-binding cassette transporter A1) CLIA Kit is specifically designed for the quantitative detection of rat ABCA1 levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ABCA1 is an essential protein involved in cholesterol efflux and lipid metabolism, playing a crucial role in maintaining cellular lipid homeostasis.
Dysregulation of ABCA1 has been linked to various metabolic disorders, including atherosclerosis and cardiovascular diseases, showcasing its importance as a biomarker for studying these conditions and potential therapeutic interventions.With its reliable performance and ease of use, the Rat ABCA1 CLIA Kit is a valuable tool for researchers studying lipid metabolism, cardiovascular health, and related diseases in rat models.
Product Name:
Rat ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit
SKU:
RTES00051
Target:
Rat ABCA1 (ATP Binding Cassette Transporter A1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ABCA 1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ABCA 1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ABCA 1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ABCA 1. You can calculate the concentration of Rat ABCA 1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
88-99
100-118
Average (%)
96
94
108
1:4
Range (%)
85-100
93-111
86-99
Average (%)
92
101
92
1:8
Range (%)
102-116
86-99
93-103
Average (%)
107
93
98
1:16
Range (%)
94-106
102-118
97-109
Average (%)
100
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
102-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.89
313.02
845.83
98.38
298.67
868.72
Standard deviation
11.58
27.64
88.9
11.26
35.66
99.21
C V (%)
12.6
8.83
10.51
11.45
11.94
11.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ABCA 1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ABCA 1 in samples. No significant cross-reactivity or interference between Rat ABCA 1 and analogues was observed.
