Rat ACE (Angiotensin I Converting Enzyme) ELISA Kit
The Rat ACE (Angiotensin I-Converting Enzyme) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of ACE levels in rat serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for a variety of research applications.ACE is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance.
Dysregulation of ACE activity is associated with various cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.With its high sensitivity and accuracy, the Rat ACE ELISA Kit from Assay Genie provides researchers with a powerful tool for studying ACE levels in rat samples, advancing our understanding of cardiovascular health and disease.
Product Name:
Rat ACE (Angiotensin I Converting Enzyme) ELISA Kit
SKU:
RTES01029
Target:
Rat ACE (Angiotensin I Converting Enzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE. You can calculate the concentration of Rat ACE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
95-110
93-110
Average (%)
100
100
100
1:4
Range (%)
93-108
82-93
84-96
Average (%)
99
88
89
1:8
Range (%)
95-108
83-94
82-94
Average (%)
100
88
88
1:16
Range (%)
88-100
80-91
83-97
Average (%)
94
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
3.06
9.84
1.2
3.18
9.23
Standard deviation
0.06
0.13
0.31
0.07
0.18
0.3
C V (%)
5.45
4.25
3.15
5.83
5.66
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ACE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ACE in samples. No significant cross-reactivity or interference between Rat ACE and analogues was observed.
