The Rat Adrenergic Receptor Beta 2 (ADRB2) ELISA Kit is a reliable and accurate tool for detecting levels of the ADRB2 protein in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers researchers dependable and reproducible results for a variety of research applications.ADRB2 is a key protein involved in mediating the effects of adrenaline and other catecholamines in the body. It plays a crucial role in regulating cardiovascular function, bronchodilation, and metabolic processes.
Dysregulation of ADRB2 has been implicated in various diseases, including asthma, heart failure, and obesity, making it an important target for therapeutic interventions.By using the Rat ADRB2 ELISA Kit, researchers can gain valuable insights into the role of ADRB2 in physiological and pathological processes, paving the way for the development of novel treatment strategies and interventions. Order your kit today and take your research to the next level.
Product Name:
Rat Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit
Product Code:
RTDL00036
Size:
96 Assays
Target:
ADRb2
Synonyms:
B2AR, Beta-2 adrenoreceptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
