The Rat AGT (Angiotensinogen) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Angiotensinogen in rat biological samples. Angiotensinogen is a critical protein involved in the renin-angiotensin system and plays a key role in regulating blood pressure and fluid balance. This ELISA kit enables researchers to accurately quantify Angiotensinogen levels, providing valuable insights into the regulation of blood pressure, cardiovascular health, and kidney function. By detecting and measuring Angiotensinogen, researchers can better understand its impact on hypertension, renal health, and cardiovascular diseases. Angiotensinogen is a precursor to angiotensin, a potent vasoconstrictor that affects blood pressure regulation.
Accurate measurement of Angiotensinogen levels can help elucidate its involvement in various physiological processes and pathological conditions. The Rat AGT ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and is user-friendly, making it a valuable tool for research into the role of Angiotensinogen in cardiovascular and renal health, hypertension, and other related conditions.
Product Name:
Rat AGT (Angiotensinogen) ELISA Kit
SKU:
AEES00350
Target:
Rat AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AGT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AGT. You can calculate the concentration of Rat AGT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
87-100
97-112
Average (%)
106
92
104
1:4
Range (%)
91-103
85-96
87-98
Average (%)
97
91
92
1:8
Range (%)
93-108
82-93
88-100
Average (%)
100
87
93
1:16
Range (%)
91-104
80-89
83-95
Average (%)
96
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
96-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.34
511.57
1458.98
190.8
480.11
1580.9
Standard deviation
10.5
25.89
51.79
12.1
19.44
57.07
C V (%)
5.32
5.06
3.55
6.34
4.05
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat AGT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AGT in samples. No significant cross-reactivity or interference between Rat AGT and analogues was observed.
