The Rat AHR (Aryl Hydrocarbon Receptor) CLIA Kit is a reliable and accurate tool for detecting and quantifying AHR levels in rat serum, plasma, and tissue samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Aryl Hydrocarbon Receptor (AHR) is a key transcription factor involved in mediating the effects of environmental toxins and regulating gene expression. Its role in cellular responses to pollutants, carcinogens, and endogenous ligands makes it a valuable biomarker for studying toxicology, cancer biology, and immune system function.
With the Rat AHR CLIA Kit, researchers can gain valuable insights into the mechanisms of AHR signaling pathways and their impact on health and disease. Its ease of use and reliable performance make it an essential tool for investigating the role of AHR in physiological and pathological processes in rat models.
Product Name:
Rat AhR (Aryl Hydrocarbon Receptor) CLIA Kit
SKU:
RTES00047
Target:
Rat AhR (Aryl Hydrocarbon Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AhR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AhR. You can calculate the concentration of Rat AhR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
88-101
93-108
Average (%)
107
95
100
1:4
Range (%)
85-97
99-110
102-116
Average (%)
91
104
110
1:8
Range (%)
99-115
96-109
84-97
Average (%)
105
102
91
1:16
Range (%)
91-105
101-117
89-102
Average (%)
98
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.28
452.43
1658.42
220.53
429.08
1823.82
Standard deviation
23.06
42.21
180.93
24.13
48.27
121.47
C V (%)
11.4
9.33
10.91
10.94
11.25
6.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AhR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AhR in samples. No significant cross-reactivity or interference between Rat AhR and analogues was observed.
