Rat AIF1 (Allograft Inflammatory Factor 1) ELISA Kit
The Rat AIF1 (Allograft Inflammatory Factor 1) ELISA Kit is specifically designed for the accurate detection of AIF1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.AIF1 is a key protein involved in inflammatory responses and immune regulation, making it an important biomarker for studying conditions related to inflammation and immune dysfunction.
By accurately quantifying AIF1 levels, researchers can gain valuable insights into the pathogenesis of diseases such as autoimmune disorders, transplant rejection, and chronic inflammation.With its reliable performance and ease of use, the Rat AIF1 ELISA Kit from AssayGenie is an essential tool for researchers looking to explore the role of AIF1 in various biological processes and disease states. Upgrade your research capabilities today with this innovative ELISA kit.
Product Name:
Rat AIF1 (Allograft Inflammatory Factor 1) ELISA Kit
SKU:
RTES00915
Target:
Rat AIF1 (Allograft Inflammatory Factor 1) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat 1AIF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat 1AIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat 1AIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat 1AIF1. You can calculate the concentration of Rat 1AIF1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
95-107
89-100
Average (%)
104
100
95
1:4
Range (%)
88-104
85-97
88-103
Average (%)
95
90
95
1:8
Range (%)
90-100
81-93
81-94
Average (%)
95
88
87
1:16
Range (%)
89-102
87-99
88-99
Average (%)
95
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.48
189.34
932.88
104.89
200.49
939.96
Standard deviation
6.61
9.43
42.73
6.78
9.84
48.03
C V (%)
6.27
4.98
4.58
6.46
4.91
5.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat 1AIF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat 1AIF1 in samples. No significant cross-reactivity or interference between Rat 1AIF1 and analogues was observed.
