The Rat ALB (Albumin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of albumin levels in rat serum, plasma, and cell culture supernatants. Albumin is a vital protein involved in various physiological functions, such as maintaining osmotic pressure and transporting drugs and hormones. Changes in albumin levels can indicate liver or kidney dysfunction, dehydration, or malnutrition.This CLIA kit offers reliable and reproducible results, making it suitable for a wide range of research applications in the fields of toxicology, pharmacology, and physiology.
Monitoring albumin levels in rat models can provide valuable insights into disease progression, drug metabolism, and treatment efficacy.For researchers studying liver diseases, kidney disorders, or drug-induced toxicity in rats, the Rat ALB CLIA Kit is an essential tool for accurate and efficient albumin quantification. Its user-friendly protocol and rapid assay time ensure convenience and efficiency in the laboratory setting.
Product Name:
Rat ALB (Albumin) CLIA Kit
SKU:
RTES00196
Target:
Rat ALB (Albumin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 μg/mL
Detection range:
0.63-40 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ALB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ALB. You can calculate the concentration of Rat ALB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
94-106
95-107
Average (%)
109
101
101
1:4
Range (%)
85-98
103-121
88-103
Average (%)
90
110
95
1:8
Range (%)
101-114
97-114
97-109
Average (%)
107
104
102
1:16
Range (%)
93-107
90-103
92-109
Average (%)
98
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.07
4.86
19.96
2.15
4.77
19.73
Standard deviation
0.26
0.4
1.67
0.19
0.55
1.44
C V (%)
12.56
8.23
8.37
8.84
11.53
7.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ALB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed.
