Rat Aldolase B, Fructose Bisphosphate (ALDOB) ELISA Kit
The Rat Aldolase B (Fructose Bisphosphate Aldob) ELISA Kit is a powerful tool for the quantitative measurement of Aldolase B levels in rat samples including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Aldolase B, also known as Fructose Bisphosphate Aldob, is a key enzyme in the glycolytic pathway and is essential for the metabolism of fructose.
Dysregulation of Aldolase B has been linked to metabolic disorders such as hereditary fructose intolerance. By accurately measuring Aldolase B levels, researchers can gain valuable insights into metabolic processes and potential therapeutic targets.Overall, the Rat Aldolase B (Fructose Bisphosphate Aldob) ELISA Kit is a valuable tool for studying metabolic disorders and exploring potential treatments, providing researchers with reliable and precise data for their experiments.
Product Name:
Rat Aldolase B, Fructose Bisphosphate (ALDOB) ELISA Kit
Product Code:
RTDL00058
Size:
96 Assays
Target:
ALDOB
Synonyms:
ALDO-BL, Liver-type aldolase
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
