The Rat Alpha-Glu Alpha-Glucosidase CLIA Kit is a reliable and accurate tool for detecting levels of alpha-glucosidase in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Alpha-glucosidase is an important enzyme involved in carbohydrate metabolism, specifically in breaking down complex sugars into simpler forms for absorption. Dysregulation of this enzyme has been associated with metabolic disorders such as diabetes and Pompe disease, highlighting its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions.
With its user-friendly protocol and high-performance characteristics, the Rat Alpha-Glu Alpha-Glucosidase CLIA Kit is a valuable tool for researchers looking to investigate the role of alpha-glucosidase in health and disease in rat models.
Product Name:
Rat alpha-Glu (Alpha Glucosidase) CLIA Kit
SKU:
RTES00599
Target:
Rat alpha-Glu (Alpha Glucosidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat α-Glu. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat α-Glu and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat α-Glu, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat α-Glu. You can calculate the concentration of Rat α-Glu in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
85-96
89-103
Average (%)
108
91
94
1:4
Range (%)
97-110
97-113
86-98
Average (%)
102
103
93
1:8
Range (%)
89-103
89-103
94-111
Average (%)
94
95
101
1:16
Range (%)
89-101
103-115
97-108
Average (%)
95
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.3
9.32
1.07
2.42
8.48
Standard deviation
0.09
0.19
0.6
0.14
0.21
0.63
C V (%)
8.33
8.26
6.44
13.08
8.68
7.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat α-Glu concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat α-Glu in samples. No significant cross-reactivity or interference between Rat α-Glu and analogues was observed.
