Rat Alpha Tocopherol Transfer Protein (TTPa) ELISA Kit
The Rat Alpha-Tocopherol Transfer Protein (TTPA) ELISA Kit is a reliable and accurate tool for measuring levels of TTPA in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TTPA is a key protein responsible for the transport of alpha-tocopherol, a form of vitamin E, in the body. It plays a vital role in protecting cells from oxidative damage and boosting immune function. Research has shown that abnormalities in TTPA levels are associated with conditions such as neurodegenerative diseases, cardiovascular disorders, and age-related cognitive decline.
By using the Rat Alpha-Tocopherol Transfer Protein (TTPA) ELISA Kit, researchers can gain valuable insights into the role of TTPA in disease development and progression, as well as potential therapeutic strategies. This kit is a valuable tool for studying vitamin E metabolism and its impact on overall health and well-being.
Product Name:
Rat Alpha Tocopherol Transfer Protein (TTPa) ELISA Kit
Product Code:
RTDL01071
Size:
96 Assays
Target:
TTPa
Synonyms:
AVED, TTP1, alphaTTP, Ataxia(Friedreich-Like)With Vitamin E Deficiency
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
