The Rat Alpha1-MG (Alpha1-Microglobulin) CLIA Kit is a cutting-edge assay kit designed for the precise detection of alpha1-microglobulin levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Alpha1-microglobulin is a vital protein with diverse functions, including antioxidant properties and involvement in immune response regulation.
Its levels can be indicative of kidney dysfunction and other health conditions, making it a valuable biomarker for studying various diseases and potential therapeutic interventions.With its advanced technology and reliable performance, the Rat Alpha1-MG CLIA Kit is an indispensable tool for researchers seeking to explore the role of alpha1-microglobulin in physiological processes and pathological conditions.
Product Name:
Rat alpha1-MG (alpha1-Microglobulin) CLIA Kit
SKU:
RTES00598
Target:
Rat alpha1-MG (alpha1-Microglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat α1-MG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat α1-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat α1-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat α1-MG. You can calculate the concentration of Rat α1-MG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-119
85-96
99-118
Average (%)
110
91
107
1:4
Range (%)
88-103
100-116
89-104
Average (%)
94
107
96
1:8
Range (%)
89-105
101-117
90-105
Average (%)
96
108
97
1:16
Range (%)
88-100
86-96
93-104
Average (%)
95
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-118
107
EDTA plasma (n=5)
101-116
108
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.79
11.23
37.87
3.92
11.23
34.25
Standard deviation
0.44
1.27
3.86
0.47
1.09
3.63
C V (%)
11.61
11.31
10.19
11.99
9.71
10.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat α1-MG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat α1-MG in samples. No significant cross-reactivity or interference between Rat α1-MG and analogues was observed.
