The Rat Alpha2-AP (Alpha2-Antiplasmin) ELISA Kit is specifically designed for the precise measurement of Alpha2-Antiplasmin levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research applications.Alpha2-Antiplasmin is an important protein involved in the regulation of blood clotting, inhibiting the activity of plasmin and preventing excessive fibrinolysis. Dysregulation of Alpha2-Antiplasmin has been linked to various health conditions, including thrombosis, hemorrhage, and inflammatory disorders.
Therefore, reliable quantification of Alpha2-Antiplasmin levels is essential for understanding these diseases and potential therapeutic interventions.With its advanced technology and proven performance, the Rat Alpha2-AP ELISA Kit provides researchers with a valuable tool for studying the role of Alpha2-Antiplasmin in health and disease. Trust AssayGenie for high-quality ELISA kits that deliver accurate and reproducible results for your research needs.
Product Name:
Rat alpha2-AP (alpha2-Antiplasmin) ELISA Kit
SKU:
RTES00841
Target:
Rat alpha2-AP (alpha2-Antiplasmin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat α2-AP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat α2-AP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat α2-AP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat α2-AP. You can calculate the concentration of Rat α2-AP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
88-103
85-95
Average (%)
100
94
90
1:4
Range (%)
90-107
82-97
85-97
Average (%)
98
89
91
1:8
Range (%)
91-104
85-97
86-100
Average (%)
98
92
93
1:16
Range (%)
90-105
81-94
89-101
Average (%)
96
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.67
17.14
93.37
10.1
16.78
88.0
Standard deviation
0.61
1.0
4.72
0.67
0.86
3.78
C V (%)
5.72
5.83
5.06
6.63
5.13
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat α2-AP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat α2-AP in samples. No significant cross-reactivity or interference between Rat α2-AP and analogues was observed.
