The Rat AMH (Anti-Mullerian Hormone) CLIA Kit is specifically designed for the accurate measurement of AMH levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.AMH is a critical hormone involved in reproductive health, particularly in the regulation of ovarian function and follicle development. Monitoring AMH levels can provide valuable insight into fertility, ovarian function, and reproductive disorders in rats.
This kit is an essential tool for studying these conditions and developing potential treatments.Overall, the Rat AMH CLIA Kit from Assay Genie is a valuable asset for researchers investigating reproductive health and fertility in rat models, providing accurate and consistent results for their experiments.
Product Name:
Rat AMH (Anti-Mullerian Hormone) CLIA Kit
SKU:
RTES00396
Target:
Rat AMH (Anti-Mullerian Hormone)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AMH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AMH. You can calculate the concentration of Rat AMH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
95-113
88-102
Average (%)
94
103
94
1:4
Range (%)
92-108
90-105
88-103
Average (%)
99
98
95
1:8
Range (%)
93-104
92-105
91-108
Average (%)
99
99
99
1:16
Range (%)
102-114
86-98
99-111
Average (%)
107
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
99-111
104
Cell culture media (n=5)
100-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.33
195.4
990.02
106.12
191.42
1043.02
Standard deviation
10.04
14.23
109.79
10.03
20.85
109.0
C V (%)
10.11
7.28
11.09
9.45
10.89
10.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AMH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AMH in samples. No significant cross-reactivity or interference between Rat AMH and analogues was observed.
