The Rat Anti-Mullerian Hormone (AMH) ELISA Kit is a specialized assay designed to measure and quantify AMH levels in various biological samples obtained from rat subjects. Anti-Mullerian Hormone serves as a crucial marker involved in the regulation of sex determination and in the development of reproductive organs, making it an essential parameter for the study of reproductive physiology and disorders. This ELISA kit enables researchers to accurately assess and analyze AMH concentrations, providing valuable insights into reproductive function, fertility, and disorders such as polycystic ovary syndrome (PCOS) and conditions related to disorders of sex development (DSD).
Accurate measurement of AMH levels using this kit is essential for understanding its role in reproductive health and disorders affecting the male and female reproductive systems. Manufactured under stringent quality control standards, the Rat AMH ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Researchers can rely on this kit to deliver robust performance, precise measurements, and ease of use, making it an excellent choice for investigations into reproductive biology and related research areas.
Product Name:
Rat AMH (Anti-Mullerian Hormone) ELISA Kit
SKU:
AEES00536
Target:
Rat AMH (Anti-Mullerian Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AMH. You can calculate the concentration of Rat AMH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
93-107
88-97
Average (%)
92
100
91
1:4
Range (%)
87-99
89-102
87-97
Average (%)
92
94
90
1:8
Range (%)
90-102
94-110
99-109
Average (%)
94
102
103
1:16
Range (%)
87-98
91-105
86-98
Average (%)
92
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
95
EDTA plasma (n=5)
90-99
95
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
164.78
488.04
1854.15
191.52
482.82
1632.48
Standard deviation
9.72
23.91
79.73
11.17
21.82
78.2
C V (%)
5.9
4.9
4.3
5.83
4.52
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat AMH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AMH in samples. No significant cross-reactivity or interference between Rat AMH and analogues was observed.
