The Rat ANG (Angiogenin) CLIA Kit is a cutting-edge tool for the accurate quantification of angiogenin levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it an invaluable asset for a diverse array of research endeavors.Angiogenin is a vital player in the process of angiogenesis, facilitating the growth of new blood vessels and influencing cell growth. Its pivotal role in various health conditions, such as cancer, cardiovascular diseases, and neurodegenerative disorders, underscores its importance as a biomarker for studying disease mechanisms and developing potential therapeutic interventions.
With the Rat ANG CLIA Kit, researchers can confidently investigate the role of angiogenin in disease pathogenesis and explore its potential as a therapeutic target. Trust in this advanced kit to provide you with the reliable data you need to advance your research efforts in the field of angiogenesis and beyond.
Product Name:
Rat ANG (Angiogenin) CLIA Kit
SKU:
RTES00388
Target:
Rat ANG (Angiogenin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ANG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ANG. You can calculate the concentration of Rat ANG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
90-104
85-97
Average (%)
108
98
91
1:4
Range (%)
101-118
86-101
93-105
Average (%)
108
93
99
1:8
Range (%)
89-105
95-111
94-106
Average (%)
97
102
101
1:16
Range (%)
95-110
100-115
95-108
Average (%)
102
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.41
110.27
448.96
43.85
110.76
448.53
Standard deviation
6.14
11.83
38.16
5.34
10.12
28.3
C V (%)
12.68
10.73
8.5
12.18
9.14
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ANG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ANG in samples. No significant cross-reactivity or interference between Rat ANG and analogues was observed.
