The Rat ANG2 (Angiopoietin 2) CLIA Kit is a cutting-edge assay designed for the quantitative measurement of Angiopoietin 2 levels in rat samples. This kit provides exceptional sensitivity and specificity, delivering accurate and consistent results for various research applications.Angiopoietin 2 is a key regulator of angiogenesis, playing a crucial role in blood vessel development and endothelial cell function. Dysregulation of Angiopoietin 2 has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a potential biomarker for disease progression and therapeutic targets.
With its advanced technology and precision performance, the Rat ANG2 CLIA Kit is a valuable tool for studying angiogenesis-related processes and exploring novel treatment strategies in a preclinical rat model. Trust in this kit to unlock new insights and drive forward your research endeavors.
Product Name:
Rat ANG2 (Angiopoietin 2) CLIA Kit
SKU:
RTES00073
Target:
Rat ANG2 (Angiopoietin 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ANG2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ANG2. You can calculate the concentration of Rat ANG2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
95-109
93-108
Average (%)
109
103
99
1:4
Range (%)
87-103
90-104
85-98
Average (%)
94
95
90
1:8
Range (%)
95-110
90-102
90-103
Average (%)
101
96
96
1:16
Range (%)
103-116
92-107
103-120
Average (%)
109
100
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
97-114
104
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.22
123.32
456.7
53.68
121.62
435.9
Standard deviation
6.43
14.02
40.19
4.98
10.5
43.98
C V (%)
12.31
11.37
8.8
9.28
8.63
10.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ANG2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ANG2 in samples. No significant cross-reactivity or interference between Rat ANG2 and analogues was observed.
