Rat Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit
The Rat ANGPTL2 (Angiopoietin-Like Protein 2) ELISA Kit is a highly reliable assay designed for the precise quantification of ANGPTL2 levels in rat serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ANGPTL2 is a key player in various physiological processes, including inflammation, lipid metabolism, and insulin sensitivity. Its dysregulation has been implicated in the pathogenesis of metabolic disorders, cardiovascular diseases, and cancer, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
With its user-friendly protocol and robust performance, the Rat ANGPTL2 ELISA Kit is an essential tool for researchers studying the role of ANGPTL2 in health and disease, offering reliable data to advance scientific understanding and drug development efforts.
Product Name:
Rat Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit
Product Code:
RTDL00075
Size:
96 Assays
Target:
ANGPTL2
Synonyms:
HARP, ARP2, Angiopoietin-Related Protein 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
