The Rat APOB (Apolipoprotein B) ELISA Kit is specifically designed for the precise measurement of apolipoprotein B levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Apolipoprotein B is a key protein involved in lipid metabolism and transportation, playing a crucial role in cholesterol homeostasis and cardiovascular health.
Abnormal levels of apolipoprotein B are associated with a range of metabolic disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Rat APOB ELISA Kit provides researchers with a reliable tool to investigate the impact of apolipoprotein B on health and disease in rat models, offering valuable insights into metabolic and cardiovascular disorders.
Product Name:
Rat ApoB (Apolipoprotein B) ELISA Kit
SKU:
RTES00962
Target:
Rat ApoB (Apolipoprotein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoB. You can calculate the concentration of Rat ApoB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
88-101
92-106
Average (%)
104
94
97
1:4
Range (%)
94-107
81-93
96-109
Average (%)
101
86
103
1:8
Range (%)
96-110
85-94
97-108
Average (%)
101
89
102
1:16
Range (%)
95-107
82-96
94-108
Average (%)
101
89
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.9
273.5
849.5
93.3
269.6
867.2
Standard deviation
4.7
14.2
44.2
6.4
12.4
42.5
C V (%)
5.06
5.19
5.2
6.86
4.6
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ApoB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoB in samples. No significant cross-reactivity or interference between Rat ApoB and analogues was observed.
