The Rat APOE (Apolipoprotein E) ELISA Kit is specifically designed for the accurate measurement of APOE levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for precise and consistent results, making it perfect for various research purposes.APOE is an important protein involved in lipid metabolism and transport, playing a critical role in the development of cardiovascular diseases and neurodegenerative disorders.
By measuring APOE levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.Overall, the Rat APOE ELISA Kit from Assay Genie is a valuable tool for studying the role of APOE in different disease states and exploring potential treatment options.
Product Name:
Rat ApoE (Apolipoprotein E) ELISA Kit
SKU:
RTES00971
Target:
Rat ApoE (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoE. You can calculate the concentration of Rat ApoE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
85-99
91-106
Average (%)
105
92
99
1:4
Range (%)
96-109
85-98
95-108
Average (%)
102
90
101
1:8
Range (%)
94-108
89-102
99-114
Average (%)
100
94
105
1:16
Range (%)
96-109
83-96
95-107
Average (%)
103
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
83-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.7
25.8
87.0
10.4
23.3
85.8
Standard deviation
0.6
1.5
3.5
0.7
1.1
4.0
C V (%)
6.19
5.81
4.02
6.73
4.72
4.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ApoE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoE in samples. No significant cross-reactivity or interference between Rat ApoE and analogues was observed.
