The Rat AST (Aspartate Aminotransferase) ELISA Kit is a sophisticated assay meticulously crafted for the specific and quantitative determination of Aspartate Aminotransferase levels in diverse rat biological samples. Aspartate Aminotransferase is a critical enzyme involved in amino acid metabolism and the transfer of amino groups, playing a key role in cellular metabolism and the functioning of multiple organs, particularly the liver and heart. Accurate measurement of Aspartate Aminotransferase through this ELISA kit provides valuable insights into liver function, cardiac health, and general metabolic activities, offering researchers a precise tool to explore the enzyme's involvement in health and disease.
This ELISA kit ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results essential for investigating Aspartate Aminotransferase levels in various physiological and pathological conditions. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for research studies aimed at unraveling the complexities of amino acid metabolism and the role of Aspartate Aminotransferase in biological processes. Trust the Rat AST ELISA Kit to enhance your research capabilities and deepen your understanding of this crucial enzyme's functions in both normal physiology and disease states.
Product Name:
Rat AST (Aspartate Aminotransferase) ELISA Kit
SKU:
AEES00317
Target:
Rat AST (Aspartate Aminotransferase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AST. You can calculate the concentration of Rat AST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
99-115
97-110
Average (%)
96
106
103
1:4
Range (%)
93-107
85-99
89-102
Average (%)
98
91
94
1:8
Range (%)
89-103
84-96
84-95
Average (%)
95
90
90
1:16
Range (%)
91-105
80-94
85-99
Average (%)
96
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
1.68
7.93
0.97
1.59
7.33
Standard deviation
0.05
0.08
0.33
0.06
0.08
0.34
C V (%)
5.32
4.76
4.16
6.19
5.03
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat AST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AST in samples. No significant cross-reactivity or interference between Rat AST and analogues was observed.
