Description
Rat ATGL (Adipose Triglyceride Lipase) ELISA Kit
The Rat ATGL (Adipose Triglyceride Lipase) ELISA Kit is a specialized assay designed for the quantitative analysis of ATGL levels in various rat biological samples. Adipose triglyceride lipase (ATGL) is a key enzyme involved in the hydrolysis of stored triglycerides in adipose tissue, playing a crucial role in lipid metabolism and energy homeostasis. Accurate measurement of ATGL levels using this ELISA kit is essential for investigating the regulation of lipid metabolism, adipocyte function, and energy balance. Dysregulation of ATGL activity has been associated with metabolic disorders, obesity, and related health conditions, making it a significant target in the study of metabolic pathways and potential therapeutic interventions.
Assay Genie's Rat ATGL ELISA Kit offers exceptional sensitivity and specificity, enabling reliable and reproducible results. Manufactured to high-quality standards, this kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers studying lipid metabolism, adipose tissue biology, and metabolic diseases.
| Product Name: | Rat ATGL (Adipose Triglyceride Lipase) ELISA Kit |
| Product Code: | AEES00401 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.78-50 ng/mL |
| Sensitivity: | 0.47 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat ATGL in samples. No significant cross-reactivity or interference between Rat ATGL and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat ATGL concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ATGL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ATGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ATGL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ATGL. You can calculate the concentration of Rat ATGL in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
