Rat AVPR1A (Arginine Vasopressin Receptor 1A) CLIA Kit
The Rat AVPR1A (Arginine Vasopressin Receptor 1a) CLIA Kit is specially designed for the precise measurement of AVPR1A levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.AVPR1A is an important receptor involved in the regulation of vasopressin, a hormone that plays a crucial role in water balance, blood pressure regulation, and social behavior.
Dysregulation of AVPR1A has been linked to various physiological and behavioral disorders, making it a valuable target for research in areas such as neuroscience, endocrinology, and pharmacology.With its reliable performance and broad applicability, the Rat AVPR1A CLIA Kit is an essential tool for investigating the function of AVPR1A and its potential implications in various biological processes and disease states.
Product Name:
Rat AVPR1A (Arginine Vasopressin Receptor 1A) CLIA Kit
SKU:
RTES00046
Target:
Rat AVPR1A (Arginine Vasopressin Receptor 1A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 ng/mL
Detection range:
93.75-6000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Av pr1a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Av pr1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Av pr1a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Av pr1a. You can calculate the concentration of Rat Av pr1a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
98-112
96-110
Average (%)
108
105
101
1:4
Range (%)
99-112
99-115
102-120
Average (%)
105
106
109
1:8
Range (%)
94-108
97-114
101-114
Average (%)
101
105
108
1:16
Range (%)
93-107
94-108
98-114
Average (%)
98
101
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
333.06
672.13
2147.47
361.89
643.0
2277.62
Standard deviation
33.77
54.71
205.73
41.0
48.03
166.95
C V (%)
10.14
8.14
9.58
11.33
7.47
7.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Av pr1a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Av pr1a in samples. No significant cross-reactivity or interference between Rat Av pr1a and analogues was observed.
