The Rat B-cell Leukemia/Lymphoma-2 (BCL2) ELISA Kit is a powerful tool for analyzing levels of BCL2 in rat samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it a valuable asset for various research endeavors.BCL2 is a critical protein involved in regulating apoptosis and cell survival, playing a key role in various diseases such as leukemia, lymphoma, and other cancers.
By accurately measuring BCL2 levels, researchers can gain valuable insights into disease progression and therapeutic interventions, ultimately advancing our understanding and treatment of these complex conditions.Overall, the Rat BCL2 ELISA Kit offers a reliable and efficient solution for studying BCL2 expression in rat models, paving the way for innovative discoveries and potential therapeutic strategies.
Product Name:
Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit
Product Code:
RTDL00135
Size:
96 Assays
Target:
Bcl2
Synonyms:
B-Cell CLL/Lymphoma 2
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
91-102
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
