The Rat BCL-2 (B-cell leukemia/lymphoma-2) CLIA Kit is a powerful tool for accurately measuring levels of BCL-2 in rat samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable asset for a variety of research studies.BCL-2 is a key regulator of apoptosis, playing a critical role in maintaining cell survival and preventing programmed cell death.
Dysregulation of BCL-2 is associated with various diseases, including cancer and autoimmune disorders, highlighting its importance as a biomarker in understanding disease pathology and potential therapeutic targets.Overall, the Rat BCL-2 CLIA Kit offers researchers a precise and efficient method to investigate the role of BCL-2 in disease mechanisms and explore new avenues for targeted treatments.
Product Name:
Rat BCL-2 (B-Cell Leukemia/Lymphoma 2) CLIA Kit
SKU:
RTES00058
Target:
Rat BCL-2 (B-Cell Leukemia/Lymphoma 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BCL-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BCL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BCL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BCL-2. You can calculate the concentration of Rat BCL-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
86-101
96-111
Average (%)
105
93
102
1:4
Range (%)
96-112
98-111
99-113
Average (%)
102
106
106
1:8
Range (%)
97-111
95-112
87-99
Average (%)
103
102
92
1:16
Range (%)
90-106
85-100
90-101
Average (%)
97
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.26
255.94
926.05
100.09
233.66
838.67
Standard deviation
8.4
26.34
101.4
10.27
19.77
54.35
C V (%)
9.01
10.29
10.95
10.26
8.46
6.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BCL-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BCL-2 in samples. No significant cross-reactivity or interference between Rat BCL-2 and analogues was observed.
