The Rat Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Kit is a specialized assay meticulously designed to quantitatively measure and analyze the levels of Bcl-2 in various biological samples from rats. Bcl-2, a critical anti-apoptotic protein, is known for its pivotal role in regulating programmed cell death and maintaining cellular homeostasis. Dysregulation in Bcl-2 expression has been linked to numerous diseases, including cancer and autoimmune disorders, emphasizing the significance of precise quantification of Bcl-2 levels in research studies. This ELISA kit boasts exceptional sensitivity and specificity, ensuring the generation of accurate and reproducible data, thereby enabling researchers to delve deeper into the intricate molecular mechanisms modulated by Bcl-2.
Manufactured under stringent quality control standards, the Rat Bcl-2 ELISA Kit guarantees robust performance and user-friendly operation, making it an optimal choice for researchers investigating the functions and therapeutic potential of Bcl-2 in diverse biological processes and disease pathologies. Trust in this kit to drive your research forward and unlock valuable insights into the role of Bcl-2 in cellular health and disease.
Product Name:
Rat Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Kit
SKU:
AEES00322
Target:
Rat Bcl-2 (B-cell Lymphoma/Leukemia 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Bcl-2. You can calculate the concentration of Rat Bcl-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
89-106
98-111
Average (%)
94
97
105
1:4
Range (%)
99-116
82-96
95-106
Average (%)
106
88
101
1:8
Range (%)
94-108
87-98
95-110
Average (%)
102
93
101
1:16
Range (%)
96-112
87-99
91-105
Average (%)
104
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.8
0.5
1.4
4.0
Standard deviation
0.03
0.06
0.17
0.03
0.07
0.2
C V (%)
6
4.29
4.47
6.0
5.0
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Bcl-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Bcl-2 in samples. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed.
