The Rat Beta-EPR (Beta-endorphin receptor) CLIA Kit (RTES00062) is a cutting-edge assay designed for the precise detection of Beta-endorphin receptor levels in rat samples. This kit boasts high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.The Beta-endorphin receptor is a key protein involved in regulating pain perception and mood, making it crucial in understanding conditions such as pain management, addiction, and mental health disorders.
By accurately measuring Beta-endorphin receptor levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop targeted therapies.Overall, the Rat Beta-EPR (Beta-endorphin receptor) CLIA Kit (RTES00062) is a valuable tool for researchers looking to study the role of Beta-endorphin receptor in various physiological and pathological processes in rats, ultimately leading to advancements in the field of neuroscience and drug development.
Product Name:
Rat beta-EPR (Beta-Endorphin Receptor) CLIA Kit
SKU:
RTES00062
Target:
Rat beta-EPR (Beta-Endorphin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat β-EPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat β-EPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat β-EPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat β-EPR. You can calculate the concentration of Rat β-EPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
100-112
89-104
Average (%)
104
106
96
1:4
Range (%)
87-100
84-97
97-113
Average (%)
92
90
103
1:8
Range (%)
99-113
91-106
97-113
Average (%)
106
98
105
1:16
Range (%)
87-98
96-109
86-98
Average (%)
93
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
96-114
104
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.08
67.0
399.36
40.3
72.45
417.74
Standard deviation
3.7
6.3
42.65
4.41
7.5
33.75
C V (%)
9.98
9.4
10.68
10.94
10.35
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat β-EPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat β-EPR in samples. No significant cross-reactivity or interference between Rat β-EPR and analogues was observed.
