Description
Rat BID (BH3 Interacting Domain Death Agonist) ELISA Kit
The Rat BID (BH3 Interacting Domain Death Agonist) ELISA Kit is meticulously crafted for the precise quantification of BH3 Interacting Domain Death Agonist levels in a variety of biological samples of rat origin. BID, a pivotal member of the Bcl-2 protein family, serves as a crucial player in the regulation of mitochondrial apoptosis pathways.
By modulating cell death processes, BID contributes significantly to cellular homeostasis, stress response, and the fine-tuning of apoptosis. Understanding the dynamics of BID expression is essential for unraveling its pivotal role in apoptotic signaling cascades and cellular fate determination. This ELISA kit offers unparalleled sensitivity and specificity, ensuring the generation of reliable and reproducible results in diverse research settings. Manufactured under rigorous quality control standards, the Rat BID ELISA Kit assures robust and high-performance outcomes, making it an indispensable tool for researchers exploring the intricate mechanisms of apoptosis, cell survival, and disease pathology. Uncover the intricate regulatory roles of BID in cellular fate decisions with confidence and precision using this advanced ELISA kit.
| Product Name: | Rat BID (BH3 Interacting Domain Death Agonist) ELISA Kit |
| Product Code: | AEES00325 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat BID in samples. No significant cross-reactivity or interference between Rat BID and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat BID concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BID. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BID and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BID, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BID. You can calculate the concentration of Rat BID in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BID were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BID were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.00 | 2.77 | 7.28 | 1.09 | 2.74 | 7.60 |
| Standard deviation | 0.06 | 0.16 | 0.28 | 0.06 | 0.12 | 0.35 |
| CV (%) | 6.00 | 5.78 | 3.85 | 5.50 | 4.38 | 4.61 |
Recovery
The recovery of Rat BID spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 94 |
| EDTA plasma (n=8) | 91-106 | 98 |
| Cell culture media (n=8) | 96-106 | 101 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
