The Rat BLC (B Lymphocyte Chemoattractant) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of BLC levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.BLC is a chemokine that plays a crucial role in the recruitment of B lymphocytes to sites of inflammation and infection. It is involved in immune responses and has been implicated in various inflammatory and autoimmune diseases.
This kit can help researchers better understand the role of BLC in these conditions and aid in the development of potential therapies.Overall, the Rat BLC CLIA Kit is a valuable tool for studying the function of BLC in immune responses and inflammatory diseases, providing researchers with a reliable and accurate method for detecting BLC levels in rat samples.
Product Name:
Rat BLC (B-Lymphocyte Chemoattractant) CLIA Kit
SKU:
RTES00065
Target:
Rat BLC (B-Lymphocyte Chemoattractant)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BLC. You can calculate the concentration of Rat BLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
87-101
95-111
Average (%)
105
94
103
1:4
Range (%)
94-107
89-104
94-111
Average (%)
101
95
101
1:8
Range (%)
94-110
89-102
101-119
Average (%)
101
95
109
1:16
Range (%)
87-102
104-119
101-117
Average (%)
93
110
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.18
41.68
249.24
22.74
45.19
251.94
Standard deviation
3.05
4.2
21.09
2.29
3.88
18.44
C V (%)
12.61
10.08
8.46
10.07
8.59
7.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BLC in samples. No significant cross-reactivity or interference between Rat BLC and analogues was observed.
