The Rat BMP-1 (Bone Morphogenetic Protein-1) CLIA Kit is a cutting-edge assay designed for the precise measurement of BMP-1 levels in rat samples. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BMP-1 is a key regulator of bone formation and plays a crucial role in skeletal development and homeostasis. Dysregulation of BMP-1 has been implicated in a range of musculoskeletal disorders, making it a valuable biomarker for studying bone metabolism and potential therapeutic interventions.
With its advanced technology and reliable performance, the Rat BMP-1 CLIA Kit is an invaluable tool for researchers investigating bone-related diseases and exploring new treatment strategies. Trust in this kit to deliver precise and reliable results for your research needs.
Product Name:
Rat BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit
SKU:
RTES00067
Target:
Rat BMP-1 (Bone Morphogenetic Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-1. You can calculate the concentration of Rat BMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
96-111
97-112
Average (%)
92
102
104
1:4
Range (%)
98-113
98-112
96-113
Average (%)
106
103
104
1:8
Range (%)
96-109
97-110
92-108
Average (%)
102
103
100
1:16
Range (%)
100-114
98-111
86-101
Average (%)
108
104
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.19
76.89
284.17
45.12
79.27
269.14
Standard deviation
3.7
6.87
29.95
5.3
8.98
18.06
C V (%)
8.37
8.93
10.54
11.75
11.33
6.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-1 in samples. No significant cross-reactivity or interference between Rat BMP-1 and analogues was observed.
