Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
The Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit is a specialized assay designed for the quantitative detection of BMP-2 levels in rat samples. BMP-2 is a crucial protein of the transforming growth factor superfamily, known for its pivotal role in bone formation and regeneration processes. This ELISA kit offers high sensitivity and specificity, allowing accurate measurement of BMP-2 levels in rat biological samples.
Detection of BMP-2 is essential for studying bone development, homeostasis, and repair mechanisms, making this kit a valuable tool for research in orthopedics, regenerative medicine, and developmental biology. Manufactured under strict quality control measures, this ELISA kit ensures consistent and reliable results, providing a reliable platform for investigating the physiological functions and therapeutic potentials of BMP-2 in different biological contexts.
Product Name:
Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
SKU:
AEES00286
Target:
Rat BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-2. You can calculate the concentration of Rat BMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
86-99
84-95
Average (%)
92
93
90
1:4
Range (%)
94-110
88-99
95-109
Average (%)
100
94
100
1:8
Range (%)
99-114
86-101
93-109
Average (%)
104
93
100
1:16
Range (%)
99-116
81-92
94-107
Average (%)
106
86
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.2
368.6
1622.5
178.6
372.2
1493.3
Standard deviation
11.1
15.5
87.6
9.5
21.2
76.2
C V (%)
6.09
4.21
5.4
5.32
5.7
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat BMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-2 in samples. No significant cross-reactivity or interference between Rat BMP-2 and analogues was observed.
