The Rat BMP-3 (Bone Morphogenetic Protein-3) CLIA Kit is specially designed for the accurate measurement of BMP-3 levels in rat samples including serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, providing precise and consistent results that are suitable for a variety of research applications.BMP-3 is a key protein that plays a crucial role in bone and cartilage development, as well as in tissue regeneration. It is involved in regulating cell differentiation and growth, making it a valuable biomarker for studying conditions related to bone health and regeneration.
By using the Rat BMP-3 CLIA Kit, researchers can accurately measure BMP-3 levels in rat samples, allowing for a better understanding of its biological functions and its potential role in various diseases and conditions. This kit is a valuable tool for investigating bone metabolism, tissue regeneration, and potential therapeutic interventions related to BMP-3.
Product Name:
Rat BMP-3 (Bone Morphogenetic Protein 3) CLIA Kit
SKU:
RTES00068
Target:
Rat BMP-3 (Bone Morphogenetic Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-3. You can calculate the concentration of Rat BMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
89-102
103-120
Average (%)
93
95
110
1:4
Range (%)
91-104
98-112
101-115
Average (%)
97
104
109
1:8
Range (%)
87-99
87-100
87-100
Average (%)
94
92
93
1:16
Range (%)
97-108
85-98
93-108
Average (%)
103
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.91
105.05
412.48
51.0
100.61
379.79
Standard deviation
4.32
8.68
40.79
5.87
10.04
35.51
C V (%)
9.21
8.26
9.89
11.51
9.98
9.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-3 in samples. No significant cross-reactivity or interference between Rat BMP-3 and analogues was observed.
