The Rat BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit is a reliable and accurate tool for measuring BMP-4 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.BMP-4 is a key signaling molecule that plays a crucial role in bone and cartilage development, as well as in the regulation of cell growth and differentiation.
Dysregulation of BMP-4 levels can contribute to various pathological conditions, including skeletal disorders and cancer progression.By using the Rat BMP-4 CLIA Kit, researchers can better understand the role of BMP-4 in various biological processes and diseases, ultimately leading to the development of novel therapeutic strategies.
Product Name:
Rat BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
SKU:
RTES00069
Target:
Rat BMP-4 (Bone Morphogenetic Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-4. You can calculate the concentration of Rat BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
88-103
93-108
Average (%)
104
95
101
1:4
Range (%)
97-109
92-104
85-96
Average (%)
103
99
91
1:8
Range (%)
99-111
88-102
98-113
Average (%)
105
96
106
1:16
Range (%)
89-104
99-114
88-101
Average (%)
95
106
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.06
68.94
230.38
28.78
64.06
220.64
Standard deviation
2.22
6.11
24.37
3.0
4.68
17.94
C V (%)
8.2
8.86
10.58
10.42
7.31
8.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-4 in samples. No significant cross-reactivity or interference between Rat BMP-4 and analogues was observed.
