The Rat BMP-5 (Bone Morphogenetic Protein-5) CLIA Kit is a reliable and accurate tool for measuring BMP-5 levels in rat samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for a variety of research applications.BMP-5 is a key protein involved in bone formation and regeneration, as well as in the development of various tissues and organs.
Dysregulation of BMP-5 has been linked to diseases such as osteoporosis, skeletal disorders, and certain cancers, highlighting its importance as a therapeutic target and biomarker for studying these conditions.With this Rat BMP-5 CLIA Kit, researchers can gain valuable insights into the role of BMP-5 in health and disease, paving the way for potential diagnostic and therapeutic advancements.
Product Name:
Rat BMP-5 (Bone Morphogenetic Protein 5) CLIA Kit
SKU:
RTES00070
Target:
Rat BMP-5 (Bone Morphogenetic Protein 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-5. You can calculate the concentration of Rat BMP-5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
100-115
98-112
Average (%)
94
106
106
1:4
Range (%)
97-111
102-117
97-115
Average (%)
102
107
105
1:8
Range (%)
102-119
102-115
86-99
Average (%)
109
109
92
1:16
Range (%)
95-111
99-115
99-112
Average (%)
102
106
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.12
284.8
670.81
103.88
283.89
720.76
Standard deviation
8.23
33.46
61.11
8.41
32.22
81.45
C V (%)
8.22
11.75
9.11
8.1
11.35
11.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-5 in samples. No significant cross-reactivity or interference between Rat BMP-5 and analogues was observed.
