The Rat BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit is specifically designed for the quantitative detection of BMP-6 levels in rat serum, plasma, and tissue homogenates. This kit offers superior sensitivity and specificity, ensuring accurate and reliable results for various research applications.BMP-6 is a vital protein that plays a key role in bone and cartilage formation, as well as in the regulation of cellular processes such as cell differentiation and proliferation. Dysregulation of BMP-6 has been linked to various skeletal disorders, making it an important biomarker for studying bone-related diseases and potential therapeutic interventions.
With the Rat BMP-6 CLIA Kit, researchers can confidently measure BMP-6 levels in rat samples, providing valuable insights into the role of this protein in physiological and pathological conditions. Its ease of use and high performance make it a valuable tool for advancing research in bone biology and related fields.
Product Name:
Rat BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit
SKU:
RTES00071
Target:
Rat BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-6. You can calculate the concentration of Rat BMP-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
92-108
102-116
Average (%)
103
99
108
1:4
Range (%)
98-113
99-114
96-111
Average (%)
106
107
103
1:8
Range (%)
99-112
87-99
90-102
Average (%)
106
92
97
1:16
Range (%)
94-110
98-115
102-119
Average (%)
101
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.78
288.7
777.34
107.93
307.15
740.3
Standard deviation
8.75
34.21
61.18
10.53
27.49
53.6
C V (%)
8.68
11.85
7.87
9.76
8.95
7.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-6 in samples. No significant cross-reactivity or interference between Rat BMP-6 and analogues was observed.
