Rat BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
The Rat BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit is meticulously designed for the precise quantitative analysis of Bone Morphogenetic Protein 6 levels in a variety of rat biological samples.
As a member of the transforming growth factor beta (TGF-β) superfamily, BMP-6 is recognized for its pivotal role in cellular differentiation, development, and tissue regeneration. This bone morphogenetic protein contributes significantly to osteogenesis and chondrogenesis, exerting crucial effects on bone formation and remodeling processes. Understanding the regulatory functions of BMP-6 is essential for elucidating its impact on skeletal biology and various cellular functions. With exceptional sensitivity and specificity, this ELISA kit ensures accurate and reproducible results, empowering researchers to explore the diverse roles of BMP-6 in cellular processes with confidence. Manufactured under strict quality control standards, this kit guarantees robust performance and ease of use, making it an ideal solution for investigations focused on deciphering the intricate mechanisms of bone formation, tissue regeneration, and cellular differentiation. Delve into the realm of BMP-6 biology with precision and reliability using this advanced ELISA kit.
Product Name:
Rat BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
SKU:
AEES00327
Target:
Rat BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-6. You can calculate the concentration of Rat BMP-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
91-107
97-114
Average (%)
95
98
104
1:4
Range (%)
96-107
87-101
97-113
Average (%)
101
93
103
1:8
Range (%)
100-117
86-98
94-106
Average (%)
108
91
101
1:16
Range (%)
97-112
82-93
98-111
Average (%)
103
87
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
4.5
0.5
1.1
4.1
Standard deviation
0.03
0.07
0.23
0.03
0.06
0.2
C V (%)
6
5.83
5.11
6.0
5.45
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-6 in samples. No significant cross-reactivity or interference between Rat BMP-6 and analogues was observed.
