The Rat BMP-7 (Bone Morphogenetic Protein-7) CLIA Kit is a sophisticated assay designed for the accurate quantification of BMP-7 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring precise and reproducible results that are invaluable for a wide range of research applications.BMP-7 is a critical growth factor that plays a pivotal role in bone formation, cartilage development, and kidney function. It is also involved in tissue regeneration and repair processes, making it a key player in various physiological and pathological conditions.
The Rat BMP-7 CLIA Kit allows researchers to study the role of BMP-7 in these processes, providing insights that can aid in the development of novel therapeutic strategies for bone and tissue-related disorders.Overall, the Rat BMP-7 CLIA Kit is a powerful tool for researchers seeking to explore the intricate mechanisms of BMP-7 signaling and its implications in health and disease. Its outstanding performance and versatility make it an essential component in any laboratory dedicated to advancing the field of bone biology and regenerative medicine.
Product Name:
Rat BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit
SKU:
RTES00072
Target:
Rat BMP-7 (Bone Morphogenetic Protein 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-7. You can calculate the concentration of Rat BMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
103-116
100-116
Average (%)
101
110
108
1:4
Range (%)
91-106
91-108
100-116
Average (%)
97
98
107
1:8
Range (%)
96-111
89-101
102-118
Average (%)
103
94
110
1:16
Range (%)
93-107
87-99
100-114
Average (%)
101
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.84
66.09
230.33
24.64
62.53
216.11
Standard deviation
2.54
6.27
25.91
1.97
7.48
24.01
C V (%)
11.12
9.49
11.25
8.0
11.96
11.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-7 in samples. No significant cross-reactivity or interference between Rat BMP-7 and analogues was observed.
