Description
Rat BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit
The Rat BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit enables precise quantitative analysis of Bone Morphogenetic Protein 7 levels in diverse rat biological samples.
As a member of the transforming growth factor beta (TGF-?) superfamily, BMP-7 is well-known for its essential role in various biological processes, particularly in bone formation, cell differentiation, and morphogenesis. BMP-7 plays a critical part in regulating cellular functions by influencing cell growth, development, and tissue regeneration. Understanding the expression levels and activity of BMP-7 is crucial for unraveling its impact on skeletal biology, cell signaling pathways, and organ development. This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results. It is designed to meet stringent quality standards, providing reliable performance and user-friendly operation for researchers aiming to investigate the multifaceted functions of BMP-7. Dive into the intricate world of bone morphogenetic proteins with confidence and reliability using this advanced ELISA kit.
| Product Name: | Rat BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit |
| Product Code: | AEES00328 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat BMP-7 in samples. No significant cross-reactivity or interference between Rat BMP-7 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat BMP-7 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-7. You can calculate the concentration of Rat BMP-7 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
